Distinguished McKnight University Professor
Department of Medicinal Chemistry
College of Pharmacy
Dr. Tretyakova’s laboratory is employing –omics methodologies to characterize the epigenetic effects of inflammation and cigarette smoking in the lung. She also uses cutting-edge mass spectrometry methods to characterize DNA nucleobases that are found in DNA as epigenetic marks and those that have become structurally altered by chemical reactions with carcinogens (DNA adducts). Dr. Tretyakova uses the tools of mass spectrometry and genomics to detect epigenetic changes in DNA methylation, hydroxymethylation, formylation, and carboxylation upon treatment with carcinogens and drugs. She has developed sensitive mass spectrometry based methodology for all five known epigenetic modifications of cytosine (MeC, hmC, fC, and caC) and employed RRBS/oxyRRBS methodologies to map epigenetic changes across the genome, RNA-seq to study the changes in gene expression, and mass spectrometry based proteomics to examine global changes of the proteome in response to exposure and to characterize protein modifications. She has also employed mass spectrometry-based tools to elucidate the fidelity of DNA methylating enzymes and identify protein readers of epigenetic marks of DNA and identified small molecule inhibitors of DNA demethylating enzymes. Dr. Tretyakova is an expert in mass spectrometry based proteomics and chemical epigenetics.